Objectives: Proper structure and function of mammal’s CNS is based on inborn neuronal function. Sensory experience based-development deeply affects these processes in the critical period. Some studies reveal that change in sensory experience can influence mammal’s learning and memory. The aim of this study is to investigate about effects of change in visual experience on rat’s spatial learning and memory through using MWM (Morris water maze).Materials & Methods: This experimental study was carried out on 30 Wistar male rats at 45 days old which randomly distributed in 3 groups; the CO (Control) group was in 12 light/12 dark cycle through birth to the end of the study, the LR (Light Reared) group was in complete light (24 hours) and the DR (Dark Reared) group was in complete darkness (n=10 for each group). Using MWM, the animals learned to find a hidden platform for 4 trials/day at 5 days. After removing platform, spatial memory has been tested at day 5 in one trial. Time & distance were spent in the correct quadrant measured and analyzed by two-way ANOVA test.Results: Our results indicate that in the learning stage, the CO rats spent less time and distance to find the hidden platform than the other groups (p<0.0001). There was no difference between all groups in probe trial.Conclusion: Change in visual experience impairs rat's spatial learning and spatial memory formation doesn't be influenced.